Split Luciferase Technology
Luciferase derived from Pyrearinus termitilluminans (Emerald Luc), is known to emit a brighter and more stable signal than conventional firefly luciferases. The N- and C-terminal domains of luciferase can be separated into 2 fragments, which can re-associate in cells. Utilizing this phenomenon, ProbeX has developed a split Emerald luciferase assay system where the two luciferase fragments spontaneously refold to generate a strong and stable signal when they are brought within proximity of each other (patent filed). This split luciferase complementation assay enables the detection of various types of protein-protein interactions, including GPCRs, with ease and high-sensitivity.
- Ligand; DAMGO, 10μM
- Ligand; DAMGO, 10μM／
Antagonist; Diprenorphine, 0.1μM
Pyrearinus termitilluminans and Bioluminescence
Photo Credit: Courtesy of Dr. Yoshihiro Ohmiya, The National Institute of Advanced Industrial Science and Technology (AIST)
- 2016.11.01 ProbeX's webpage renewed
- 2015.03.02 Webinar on Protein-Protein Interaction(PPI) is now available on demand!
- 2015.01.30 Upcoming Online Webinar on Protein-Protein Interaction(PPI) Detection
- 2014.12.16 Serial essay volume 10 was posted
- 2014.11.13 Serial essay volume 9 was posted
- 2014.10.10 Serial essay volume 8 was posted
- 2014.10.08 New Stable Cell Lines Released
- 2014.09.11 Serial essay volume 7 was posted