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Detection of Molecular Processes within Living Cells and Mouse by ProbeX Biosensor.

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Pseudocolor Image of the Fluorescence of GFP, obtained from the HeLa cells expressing the Biosensor.

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Pseudocolor Image of the Luciferase Luminescence by the Biosensor from the Balb/c Nude Mouse．

Original Papers

  1. Rapid and High-Sensitivity Cell Based Assays of Protein－Protein Interactions Using Split Click Beetle Luciferase Complimentation: An Approach to the Study of G-Protein Coupled Receptors. Misawa N., Kafi A. K. M., Hattori M., Miura K., Masuda K., Ozawa T., Anal. Chem. ,82, 2552-2560 (2010).
  2. Cyclic luciferase for real-time sensing of Caspase-3 activities in living mammals, Akira Kanno, Yuko Yamanaka, Hisashi Hirano, Yoshio Umezawa, and Takeaki Ozawa, Angew. Chem. Int. Ed. 46, 7595-7599 (2007).
  3. Nongenomic activity of ligands in the association of androgen receptor with Src., Sung Bae Kim, Akira Kanno, Takeaki Ozawa, Hiroaki Tao, and Yoshio Umezawa, ACS Chemical Biology, 2, 484-492 (2007).
  4. A method for determining the activities of cytokines based on the nuclear transport of nuclear factor-kappa B.. S. B. Kim, Y. Natori, T. Ozawa, Y. Umezawa, and H. Tao, Analytical Biochemistry, 359, 147-149 (2006).
  5. Intein-Mediated Reporter Gene Assay for Detecting Protein-Protein Interactions in Living Mammalian Cells. A. Kanno, T. Ozawa and Y. Umezawa, Anal. Chem., 78, No. 2, 556-560 (2006).
  6. Quantitative Determination of Protein Nuclear Transport Induced by Phosphorylation or by Proteolysis. S. B. Kim, R. Takao, T. Ozawa and Y. Umezawa, Anal. Chem., 77, No. 21, 6928-6934 (2005).
  7. A genetically encoded indicator for assaying bioactive chemicals that induce nuclear transport of glucocorticoid receptor. S. B. Kim, T. Ozawa and Y. Umezawa, Analytical Biochemistry, 347, 213-220 (2005).
  8. High-throughput sensing and non-invasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. S. B. Kim, T. Ozawa, S. Watanabe and Y. Umezawa, Proc. Natl. Acad. Sci., USA, 101, No. 32, 11542-11547 (2004).
  9. Locating a protein-protein interaction in living cells via split Renilla luciferase complementation. A. Kaihara, Y. Kawai, M. Sato, T. Ozawa and Y. Umezawa, Anal. Chem., 75, No. 16, 4176-4181 (2003).
  10. Protein Splicing-Based Reconstitution of Split Green Fluorescent Protein for Monitoring Protein-Protein Interactions in Bacteria: Improved Sensitivity and Reduced Screening Time. T. Ozawa, M. Takeuchi, A. Kaihara, M. Sato and Y. Umezawa, Anal. Chem., 73, No. 24, 5866-5874 (2001).

Reviews

  1. 1.　Genetically Encoded Optical Probes for Imaging Cellular Signaling Pathways. Y. Umezawa, Biosens. Bioelectron., 20, No. 12, 2504-2511 (2005).
  2. 2.　Genetically encoded optical probes for molecular processes in living cells. Y. Umezawa, TrAC Trends Anal. Chem., 24, No. 2, 138-146 (2005).
  3. 3.　Seeing what was unseen in biological systems. Y. Umezawa, TrAC Trends Anal. Chem., Guest Editorial, 23, No. 6, xvii (2004).

Patent

1. USP 7166447, EP 1229330, Probe for analyzing protein-protein interaction and method of analyzing protein-protein interactions with the use of the same

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